Passaging mESCs on feeder

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Revision as of 22:21, 7 May 2013 by >TinaLo (Created page with "1. Aspirate media from cells 2. Wash well once with PBS 3. Add 1ml TrpLE to each well, incubate 5 min at 37C 4. Remove TrpLE 5. Add 1ml mESC media 6. Pipette using p1000...")
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1. Aspirate media from cells

2. Wash well once with PBS

3. Add 1ml TrpLE to each well, incubate 5 min at 37C

4. Remove TrpLE

5. Add 1ml mESC media

6. Pipette using p1000 20-30 times to dissociate

7. Transfer 167ul (1:6) dissociated cells to fresh feeders (3ml total media)