Daniel:Notebook/HiResChrPaint/2013-5-8

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Revision as of 18:43, 8 May 2013 by >Djacobse
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Probe Prep Test (Started 4/22/2013)

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Column Purification vs. Ethanol Precipitation

Ethanol Precipitation (continued from 5/6/2013)

5. Centrifuged 30 min at 10000 rpm at 4C
6. Removed supernatant and added 750 uL chilled EtOH
7. Centrifuged 15 min at 10000 rpm at 4C
8. Removed supernatant and dried in hood
9. Resuspended in 10 uL nf H20

TBU Gel comparison

Protocol 2

1. Gel lanes