Daniel:Notebook/HiResChrPaint/2013-5-8
Jump to navigation
Jump to search
Probe Prep Test (Started 4/22/2013)
Column Purification vs. Ethanol Precipitation
Ethanol Precipitation (continued from 5/6/2013)
5. Centrifuged 30 min at 10000 rpm at 4C 6. Removed supernatant and added 750 uL chilled EtOH 7. Centrifuged 15 min at 10000 rpm at 4C 8. Removed supernatant and dried in hood 9. Resuspended in 10 uL nf H20
TBU Gel comparison
1. Gel lanes
Sample | 0.5 Ladder | 1 uL Ladder | Original | Column (1uL) | Column (2uL) | EtOH (1 uL) A | EtOH (2uL) A | EtOH (1 uL) B | EtOH (2uL) B |
uL sample | 0.5 | 1 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
uL TBE | 9.5 | 9 | 9.5 | 9.5 | 9.5 | 9.5 | 9.5 | 9.5 | 9.5 |
uL 2X buffer | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
Loaded | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
Dilution | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
2. Heat shock 10 minutes at 75 C 3. Run gel for 24 minutes at 250V 4. Stain with 3 uL SYBR gold for 20 minutes
Gel Results
Volume Report: File:PrimerQuant 050813.xls