Daniel:Notebook/HiResChrPaint/2013-5-10
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Probe Prep Test 2
Purpose: After extensive troubleshooting, I think I am once again ready to try creating the probes, starting from qPCR and hopefully going all the way to staining. My plan is to use only two sets, V4S3 and V6S3 as before, to help me focus better. The key thing that I've learned from the previous tries is that I need more probe. As such, I am modifying the qPCR. First of all I'm going to use, on Dr. Zhang's recommendation, the suggested protocol for qPCR using the KAPA kit. Previously I was using a different protocol that I felt was a bit short.