Daniel:Notebook/HiResChrPaint/2013-5-10

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Revision as of 15:10, 10 May 2013 by >Djacobse (Created page with "=Probe Prep Test 2= Back to Calendar Purpose: After extensive troubleshooting, I think I am once again ready to try creating the probes, s...")
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Probe Prep Test 2

Back to Calendar

Purpose: After extensive troubleshooting, I think I am once again ready to try creating the probes, starting from qPCR and hopefully
going all the way to staining.  My plan is to use only two sets, V4S3 and V6S3 as before, to help me focus better.  The key thing that I've
learned from the previous tries is that I need more probe.  As such, I am modifying the qPCR.  First of all I'm going to use, on Dr. Zhang's 
recommendation, the suggested protocol for qPCR using the KAPA kit.  Previously I was using a different protocol that I felt was a bit short.

Expansion qPCR