Daniel:Notebook/HiResChrPaint/2013-5-13
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Probe Prep Test 2 (Started 5/10/2013)
TBE Gel
1. 0.5 uL sample; 6 uL TBE, 2 uL dye per sample 2. Gel Arrangement(5-12 are digested samples that have been column purified, ready to use for lambda exo digestion):
Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Sample | 25bp Ladder | V6S3A Undigested | V4S3C Pre-Column | V6S3B Pre-Column | V4S3A | V4S3B | V4S3C | V4S3D | V6S3A | V6S3B | V6S3C | V6S3D |
3. Loaded 7 uL per sample 4. Ran gel for 30 minutes at 200V 5. Stained 2 minutes with 3 uL SYBR gold