Daniel:Notebook/HiResChrPaint/2013-5-13

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Revision as of 18:07, 13 May 2013 by >Djacobse
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Probe Prep Test 2 (Started 5/10/2013)

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TBE Gel

1. 0.5 uL sample; 6 uL TBE, 2 uL dye per sample
2. Gel Arrangement(5-12 are digested samples that have been column purified, ready to use for lambda exo digestion):
Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample 25bp Ladder V6S3A Undigested V4S3C Pre-Column V6S3B Pre-Column V4S3A V4S3B V4S3C V4S3D V6S3A V6S3B V6S3C V6S3D
3. Loaded 7 uL per sample
4. Ran gel for 30 minutes at 200V
5. Stained 2 minutes with 3 uL SYBR gold

Gel Results

File:DpnDigest 051313.jpg