Alice:LabNotes/2008-9-15
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extending the probe length
After PCR amplification after CircleHelper insertion, T7 exonuclease digestion was performed. And the detailed procedures can be found through this link: [[1]]
2D gel to take out the band we are interestd in (160bp): File:ZhangLab 2 2008-09-15 12hr 53min.jpg