Daniel:Notebook/HiResChrPaint/2013-5-20

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Revision as of 21:35, 20 May 2013 by >Djacobse
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Probe Prep Test 2 (Started 5/10/2013)

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Dye Coupling

Protocol

1. Warm reagents (bicarb,h20,DMSO) to 37C
2. Add 2.5 uL DNA and 1.5 uL nfH20 to 0.2 mL tube
3. Denature at 95C for 5 minutes and snap cool using ice box
4. Add 2.2 uL DMSO to dye, vortex ≥10 seconds
5. Add 1 uL dye to each reaction
6. Incubate in the dark for 1 hour
7. Add 5 uL 3M NaOAc and 40 uL nfH20
8. Purify using ssDNA column
9. Check using nanodrop; use microarray setting

Cells

Need to get some cells (fibroblasts, I think) from Alan.  He is checking them today but they might not be ready until Wednesday.  
If they are ready today I will be fixing them and getting ready for hybridization.