EricChu:LabNotesMDA/2013-5-21
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MDA Samples Collection from MDA Device v7
- Continued from yesterday
- Some valve lines became empty overnight
- All samples were pushed down from the chambers by TE buffer to reduce mixing
- Center 8 samples were collected first
- Bubbles were generated from empty valve lines during sample collection. Video was taken when bubbles pushed the samples away from chamber 23, and that caused mixing.
- All chambers were collected in about 6ul volume.
qPCR Verification of MDA
- qPCR result of the 2nd single MEF cell experiments is similar to the 1st experiment.
- Out of 24 samples,
- A total of 8 samples yielded over 1ng product.
- A total of 8 samples yielded 500pg-1ng product.
- 3 samples yielded 100pg-500pg and 5 samples yielded less than 100pg.
- All 8 samples of low yield (<500pg) were coming from the chambers 18-24
chamber | total (pg) |
1 | 937.82126 |
2 | 1660.2249 |
3 | 1587.6281 |
4 | 1696.0932 |
5 | 500.50544 |
6 | 548.40513 |
7 | 1025.5768 |
8 | 2111.1235 |
9 | 1805.3481 |
10 | 855.75854 |
11 | 1264.0812 |
12 | 715.56902 |
13 | 755.17198 |
14 | 762.9601 |
15 | 453.42019 |
16 | 649.84148 |
17 | 1271.5977 |
18 | 35.038787 |
19 | 11.20207 |
20 | 19.804252 |
21 | 127.08806 |
22 | 20.274461 |
23 | 110.39759 |
24 | 95.640833 |
Post-CoRE Library Construction and PCR Verification (accompany to Noi/NOTES/2013-5-22)
- 16 samples prepared by CoRE was given to