Daniel:Notebook/HiResChrPaint/2013-6-2

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Revision as of 22:11, 2 June 2013 by >Djacobse (Created page with "=Probe Prep 3 (Started 5/29/2013)= ==DpnII Digestion Test== Only running 1/4 of DNA I have for each sample to compare DpnII reac...")
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Probe Prep 3 (Started 5/29/2013)

DpnII Digestion Test

Only running 1/4 of DNA I have for each sample to compare DpnII reaction yield using aadUTP DNA and normal DNA.
1. Set up 
Sample uL sample uL Dpn II (50 U/uL) uL 10x buffer uL H20 total
alpha 4-A 35 2 5 8 50
alpha 6-A 35 2 5 8 50
beta 4-A 35 2 5 8 50
beta 6-A 35 2 5 8 50
2. Incubate in thermocycler at 37C for 2 hours; heat shock 20 minutes at 65 C