Daniel:Notebook/HiResChrPaint/2013-6-5
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Probe Prep 3 (Started 5/29/2013)
DpnII Digestion
Finishing the digest on the PCR amplified probes, aa-dUTP and no aa-dUTP. Will run for 2 hours since I didn't see any qualitative difference between the two.
1. Set up
Sample | uL sample | uL Dpn II (50 U/uL) | uL 10x buffer | uL H20 | total |
alpha 4-B | 35 | 2 | 5 | 8 | 50 |
alpha 6-B | 35 | 2 | 5 | 8 | 50 |
beta 4-B | 35 | 2 | 5 | 8 | 50 |
beta 6-B | 35 | 2 | 5 | 8 | 50 |
2. Incubate in thermocycler at 37C for 16 hours; heat shock 20 minutes at 65 C