Daniel:Notebook/HiResChrPaint/2013-6-5

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Revision as of 22:40, 5 June 2013 by >Djacobse (Created page with "=Probe Prep 3 (Started 5/29/2013)= Back to Calendar ==DpnII Digestion== Finishing the digest ...")
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Probe Prep 3 (Started 5/29/2013)

Back to Calendar

DpnII Digestion

Finishing the digest on the PCR amplified probes, aa-dUTP and no aa-dUTP.  Will run for 2 hours since I didn't see any qualitative difference
between the two.
1. Set up 
Sample uL sample uL Dpn II (50 U/uL) uL 10x buffer uL H20 total
alpha 4-B 35 2 5 8 50
alpha 6-B 35 2 5 8 50
beta 4-B 35 2 5 8 50
beta 6-B 35 2 5 8 50
2. Incubate in thermocycler at 37C for 16 hours; heat shock 20 minutes at 65 C