Hosuk:LabNotes/2013-6-10

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Revision as of 19:48, 10 June 2013 by >Hosuki78 (Created page with "=====Training Day 1===== *Some of important things *Phi29 enzyme from '''Enzymatics''' is used. *Actually Jay likes to use (or trusts) Enzymatics, so almost all reagents are f...")
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Training Day 1
  • Some of important things
  • Phi29 enzyme from Enzymatics is used.
  • Actually Jay likes to use (or trusts) Enzymatics, so almost all reagents are from Enzymatics.
  • RNase H step after RT is critical step, so do this carefully.
  • Try HeLa cells unless I get rolonies with PGP1F.