Hosuk:LabNotes/2013-6-10
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Training Day 1
- Some of important things
- Phi29 enzyme from Enzymatics is used.
- Actually Jay likes to use (or trusts) Enzymatics, so almost all reagents are from Enzymatics.
- RNase H step after RT is critical step, so do this carefully.
- Try HeLa cells unless I get rolonies with PGP1F.
- The sample (that I've got some signal at 05/29) doesn't seem to have actual rolonies,
- There are no dots, just too much even signal distribution.