Matt:LabNotes/2013-6-12

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Revision as of 00:21, 13 June 2013 by >Mzcai
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Verify Probe Capture

Primers:
CP-2-FA - 'GCACGATCCGACGGTAGTGT'
CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'

Components Volume
Circle Oligos x
100uM 0.4
100uM 0.4
2X KAPA SYBG 50
H2O x
Total 100


 Program
 98C 30sec--> (98C 10sec --> 52C 30sec --> 72C 30sec)x8 cycles --> (98C 10sec --> 60C 30sec --> 72C 30sec)x15 cycles--> 72C 3 min --> 15C hold