Blue:RNA-Seq Experiments:06032013

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Revision as of 00:44, 20 June 2013 by >B1lake (Created page with "'''Library:''' BL_mESC_RT_N2id1-96_0603 '''Purpose:''' Test run of the Mg-based single cell totoRNAseq protocol with the following modifications: (1) 1uM T20; (2) 1/5 diluted...")
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Library: BL_mESC_RT_N2id1-96_0603

Purpose: Test run of the Mg-based single cell totoRNAseq protocol with the following modifications: (1) 1uM T20; (2) 1/5 diluted PNK; (3) 20 or 50U MMLV; (4) 10uM TSO.R04; (5) PhusionHF amplification.

Outcome: Still significant issue with single stranded library amplification, but looks like undiluted enzymes/primers works better.


File:06032013.pdf