Matt:LabNotes/2013-6-26
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Page Size Selection of 0 gap probes
USER/DpnII digested 0 gap probes:
Noi made 11.36ug/400ul [1] I made 10.58ug/180ul [2]
To run ~3ug/gel, I use 100ul of probes that Noi made for each of the 4 gels
- Ran TBU gel at 220V for 30 min with samples and ladder prepared below
- Heated TBE buffer in microwave
- Denatured samples/ladder for 10 min at 75C before putting on cold rack for 1 min
- Size selected for brightest band below 200bp
Components | 1X Volume | 4X Volume |
Enzyme digested probes (~12.6ug/400ul) | 105 | 420 |
TBE-Urea Buffer 2X | 105 | 420 |
H2O | 0 | 0 |
Total | 210 | 840 |
Components | 1X Volume | 4X Volume |
10-bp DNA ladder (1 ug/ul) | 1 | 4 |
TBE-Urea Buffer 2X | 5 | 20 |
H2O | 4 | 16 |
total | 10 | 40 |