Hosuk:LabNotesMDA/2013-6-28

From ZhangLabWiki
Revision as of 17:48, 28 June 2013 by >Hosuki78 (→‎Verification with qPCR)
Jump to navigation Jump to search


MDA with PGP1F, 6th

  • MDA run for 8hr @06/27
  • 400mM ALS --> original protocol
  • Slow mixing --> 1 sec period, mixing for 5min
  • Run MDA in ring mixer --> 25th chamber!
  • Push ring mixer volume with air
    • Left 8 chamber were empty, too many empty chamber...


Verification with qPCR

  • Result looked good.
  • Most of chambers have 500pg~2400pg of amplicons.
  • Left 8 chamber group were nearly empty, so that's why ch6 has almost no amplicon was detected.
  • 25th chamber(Ring mixer) had less amount than other chamber did, but there was amplicon!

File:QPCR Result 2013-06-28 Edited.png File:QPCR Result MassTable 2013-06-28.png

CoRE fragmentation

  • Done @06/28