Matt:LabNotes/2013-6-28

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Revision as of 21:28, 28 June 2013 by >Mzcai
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Probe Capture

' 20 gap 0 gap '
Probe Size 12964 13179
gDNA HAPMAP C1 (87ng/ul) 300 ng 300 ng
gDNA MW 1.95x10^12 g/mol 1.95x10^12 g/mol
gDNA 1.5385x10^-19 mol 1.5385x10^-19 mol
Probe Required (1000:1) 1.5385x10^-16 mol 1.5385x10^-16 mol
Probe MW 8.1419x10^8 g/mol 8.2769x10^8 g/mol (Probe size x (193nt x 325 Da/nt + 79Da)
Amount Probe Required 125 ng (7.44ul) 127 ng (4.47 ul)

0 gap probes from 2013-6-27
20 gap probes from 2013-6-6
cDNA from 2013-6-26

Tube # Probe type Samples Probes (ul) 10X AmpLigase Buffer (ul) Target (ul) H2O (ul) Total
1 0 gap (28.4ng/ul) NTC 4.47 3 0 22.53 30
2 0 gap (28.4ng/ul) gDNA HAPMAP C1 (87ng/ul) 4.47 3 3.45 19.08 30
3 0 gap (28.4ng/ul) cDNA 4.47 3 20 2.53 30
4 20 gap (16.79ng/ul) NTC 7.44 3 0 19.56 30
5 20 gap (16.79ng/ul) gDNA HAPMAP C1 (87ng/ul) 7.44 3 3.45 16.11 30
6 20 gap (16.79ng/ul) cDNA 7.44 3 20 0 30.44