Hosuk:LabNotesMDA/2013-7-2
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MDA with PGP1F, 7h
- 269mM ALS for 240mM ALS in ring mixer
- Run MDA with the volume in ring mixer! --> 25th chamber
- Mixing pump : 1sec period, 5min
- Push ring mixer volume with air
- Verification of the amount of amplicon by qPCR
- Start 10:00pm 07/01
- End 09:00am 07.02 --> about 11hr
Result
- qPCR result :