Daniel:Notebook/HiResChrPaint/2013-7-11

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Revision as of 18:25, 11 July 2013 by >Djacobse
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S2 Probe Prep (Started 5/29/2013)

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ULYSIS Dye Coupling (Test 6)

Should be the last test.  I'm going to test at 15 minutes and 20 minutes incubation time, 1 uL each.  If the
results go well this morning I will be labeling the V6S2 set this afternoon. 
1. Add 1 uL DNA in 19 uL labeling buffer (Component C)
2. Denature DNA for 5 minutes at 95C; snap cool on ice
3. Add 1 uL dye to sample; add 4 uL labeling buffer
4. Incubate for 15 minutes (AmpR9) or 20 minutes (AmpR10) at 80C, snap cool on ice
5. Purify using Centri-Sep Column