Daniel:Notebook/HiResChrPaint/2013-7-15

From ZhangLabWiki
Revision as of 17:46, 15 July 2013 by >Djacobse (Created page with "=S2 Probe Prep (Started 5/29/2013)= Back to Calendar ==ARES Labeling of V6S2== Trying ARES lab...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

S2 Probe Prep (Started 5/29/2013)

Back to Calendar

ARES Labeling of V6S2

Trying ARES labeling of V6S2 set randomly primed with aa-dUTP.

V6S2 ~

  • Following [[Daniel:Protocols/AlexaDyeCouple#ARES|ARES Protocol
1. Warm DMSO and bicarbonate labeling buffer and nuclease free water to room temperature
2. Resuspend DNA in 2.5uL nfH20
3. Add 5 uL DNA and 3 uL of sodium bicarbonate buffer in PCR tube
4. Denature samples for 5 minutes at 95C, then snap cool using ice box
5. Dissolve 1 vial of reactive dye in 2 uL solvent; vortex ≥10 seconds
   *dye cannot be saved for later use.  Use immediately!
6. Add 2 uL dye to sample
7. Incubate in the dark for 1 hour
8. Add 5 uL 3M NaOAc and 35 uL nfH20 to sample
9. Purify using Centri Sep column

ULYSIS Labeling of V6S2