Daniel:Notebook/HiResChrPaint/2013-7-23
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S2 Probe Preparation
DpnII Digestion (from 7/18/2013)
3. Pool samples for V4 set into 4AB and 4CD 4. Column Purification, elute with 30 uL EB 5. Measure concentrations in nanodrop
Nanodrop Results
DpnII Digest gel
1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all)
Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Sample | 10bp ladder | V4S4A | V4S4B | V4S4C | V4S4D | V6S4A | V6S4B | V6S4C | V6S4D |