Kun:LabNotes/ASE/2007-11-1

From ZhangLabWiki
Revision as of 01:51, 2 November 2007 by KunZhang (talk | contribs) (New page: ===Exp1: Repeat PCR amplification on the eSNP84k library using NEB 2X Taq master mix=== Reactions eSNP84k set1-5 (100nM): x 8 x 5 Template 0.2ul...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Exp1: Repeat PCR amplification on the eSNP84k library using NEB 2X Taq master mix

Reactions eSNP84k set1-5 (100nM):

                              x 8 x 5 
    Template        0.2ul      1.6ul x 5 
    2x Master mix    50ul      400ul x 5
    100uM AP1V41U   0.4ul      3.2ul x 5
    100uM phosAp2V4 0.4ul      3.2ul x 5
    H2O              49ul      386ul x 5
    50X SYBR I      0.8ul      6.4ul x 5

94C 2min -> 16 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 72C 3min -> 4C hold