Hosuk:LabNotesMDA/2013-7-18

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Revision as of 18:30, 26 July 2013 by >Hosuki78 (Created page with "*LabNote ===MDA with PGP1F, 8th, Dev v9.1=== *Use new device (v9.1) ====KOH concentration for (ALS+Cell) mix==== *I...")
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MDA with PGP1F, 8th, Dev v9.1

  • Use new device (v9.1)


KOH concentration for (ALS+Cell) mix

  • In 20uL reaction, the final concentration of KOH in ALS while denaturing is 240mM.
  • In the prev version,269mM of KOH in ALS buffer has used (v7.3 ring mixer device).
  • For this v9.1 device, 286mM of KOH in ALS buffer is used (Cell : (ALS-Cell) = 1 : 5.247).