Hosuk:LabNotesMDA/2013-7-18
Jump to navigation
Jump to search
MDA with PGP1F, 8th, Dev v9.1
- Use new device (v9.1)
KOH concentration for (ALS+Cell) mix
- In 20uL reaction, the final concentration of KOH in ALS while denaturing is 240mM.
- In the v7.3 version, 269mM of KOH in ALS buffer has used (v7.3 ring mixer device).
- For this v9.1 device, 286mM of KOH in ALS buffer is used (Cell : (ALS-Cell) = 1 : 5.247).