Daniel:Notebook/HiResChrPaint/2013-8-1

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Revision as of 21:43, 1 August 2013 by >Djacobse
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Troubleshooting qPCR (Started 7/31/2013)

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PCR 2 (Me)

As per yesterday's finale, I'm trying single reaction PCR again, comparing Noi and my Master Mixes, and using the Eppendorf thermocycler instead of the BioRad one.

  20 nM OligoPool 2X Kapa SYBR qPCR MM 100 uM AP1V41U 100 uM AP2V4 100 uM AP1V61U 100 uM AP2V6 2 mM aa-dUTP H20 Total Volume
V4S1D 0.5 50 0.5 0.5 0.5 0.5 0 47.5 100
V4S1N 0.5 50 0.5 0.5 0.5 0.5 0 47.5 100

qPCR Results