Daniel:Notebook/HiResChrPaint/2013-8-5

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S1 Probe Preparation (Started 8/2/2013

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Researched this morning on new ways to improve the FISH protocol. First of all, I noticed that in the supplementary material of Beliveau et al the images are not so crystal clear as the paper (go figure). From the supplements, I see that nucleii seem to have more background than I originally suspected (see thumbnail).

For a good image, the signal should be distinctly above background, but there will likely be background present in the nucleus. Second, also in the thumbnail,



Lambda Exonuclease Digestion