Daniel:Notebook/HiResChrPaint/2013-8-10

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Revision as of 00:09, 11 August 2013 by >Djacobse (Created page with " ==Dye Coupling== *Following ULYSIS Protocol 1. Resuspend DNA in 20 uL labeling buffer (Component C) 2. Denature DNA for 5 minu...")
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Dye Coupling

1. Resuspend DNA in 20 uL labeling buffer (Component C)
2. Denature DNA for 5 minutes at 95C; snap cool on ice
3. Add 1 uL dye to sample
4. Incubate for 20 minutes at 80C, snap cool on ice
5. Purify using Centri Sep column