Daniel:Notebook/HiResChrPaint/2013-8-10
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S2 Probe Prep (ULS; Started 8/8/2013)
Dye Coupling
- Following ULYSIS Protocol
1. Resuspend DNA in 20 uL labeling buffer (Component C) 2. Denature DNA for 5 minutes at 95C; snap cool on ice 3. Add 1 uL dye to sample per ug 4. Incubate for 20 minutes at 80C, snap cool on ice 5. Purify using Centri Sep column