Daniel:Notebook/HiResChrPaint/2013-8-10

From ZhangLabWiki
Revision as of 00:10, 11 August 2013 by >Djacobse
Jump to navigation Jump to search

S2 Probe Prep (ULS; Started 8/8/2013)

Back to Calendar

Dye Coupling

1. Resuspend DNA in 20 uL labeling buffer (Component C)
2. Denature DNA for 5 minutes at 95C; snap cool on ice
3. Add 1 uL dye to sample per ug
4. Incubate for 20 minutes at 80C, snap cool on ice
5. Purify using Centri Sep column