Hosuk:LabNotesMDA/2013-8-14
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MDA with PGP1F, 12th, Dev v9.1[edit]
- Use mineral oil in valve lines
- I expect...
- No any leaking of H2O into flow channel
- Reduce cross-contamination
- But the response of valves became slower … maybe oil is compressed when pushing?
Experiment[edit]
- MDA reaction for 6 hr.
- qPCR run for verification
- Extract just almost 2uL at each tube and run qPCR.
Result[edit]
- Result : Good
- Looked more even than previous results.
- Amount of amplicons are detected more along to the right side of chambers
- Maybe the templates moved more toward right chambers?
- Would the amplicons are generated more? if the reaction time is longer...
File:QPCR Result MDAv9.1 2013-08-13 fig3 HistoryRecent.png
Next Plan[edit]
- Water in valves for cell capture and mineral oil for rest of valves
- Try do Tagmentation
- File:Water in valves for cell capture MDAv9.1 2013-08-13.png