Hosuk:LabNotesMDA/2013-8-14

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Revision as of 17:36, 14 August 2013 by >Hosuki78
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MDA with PGP1F, 12th, Dev v9.1[edit]

  • Use mineral oil in valve lines
  • I expect...
    • No any leaking of H2O into flow channel
    • Reduce cross-contamination
    • But the response of valves became slower … maybe oil is compressed when pushing?


Experiment[edit]

  • MDA reaction for 6 hr.
  • qPCR run for verification
  • Extract just almost 2uL at each tube and run qPCR.


Result[edit]

  • Result : Good
  • Looked more even than previous results.
  • Amount of amplicons are detected more along to the right side of chambers
  • Maybe the templates moved more toward right chambers?
  • Would the amplicons are generated more? if the reaction time is longer...


File:QPCR Result MDAv9.1 2013-08-13 fig3 HistoryRecent.png


Next Plan[edit]