EricChu:LabNotesMDA/2013-9-10

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Revision as of 22:27, 10 September 2013 by >Ericchu
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MDA Device v7 (PGP1 #?) with Tagmentation

  • Device preparation was described in EricChu:LabNotesMDA/2013-5-20
  • MDA recipe was followed to the protocol in EricChu:LabNotesMDA/2013-5-14
  • Compare experiments of (1) v7 device with Tagmentation and (2) v9 device with Tagmentation

MDA Master Mix Recipe for v7

Phi29 10x buffer 2.33 uL
200uM N6 primer 8.45 uL
25mM dNTP 1.688 uL
8% Tween20 2.33 uL
Phi29 (1ug/ul, High Conc. Epicentre) 0.84 uL
H2O 4.53 uL
  • Preparing "negative" control: added 0.35ul of water into 10ul MDA mastermix in PCR tube. 15 hours at 30C.

ALS, NS buffer

ALS (240mM after mixing cell capture volume)

H2O 77.12 uL
2M DTT 5 uL
5M KOH 5.38 uL
8% Tween20 12.5 uL


NS (matching ALS)

H2O 28.66 uL
NS stock 58.84 uL
8% Tween20 12.5 uL


  • NS stock : 4mL of 1M HCl + 6mL of 1M TrisHCl