Daniel:Notebook/HiResChrPaint/2013-9-11

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Revision as of 23:43, 11 September 2013 by >Djacobse
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Fibroblast Adherence Timecourse (Started 09/09/2013)

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Petri dishes are in! So I've got a revised itinerary. First, a recap. There are a few important variables to track in this experiment:

  • (1) surface type (slide or dish)
  • (2) trypsin dilution (to ensure cells may attach)
  • (3) Cell seeding density (1,000,000 cells/mL recommended)
  • (4) Adding more media to help growth

New Template for Experiment

Name Surface Trypsin Dilution Cell Seeding Density Media Kicker
Slide 09/09/13 Slide 1:10 1.00E+06 None
Slide 1 Slide 1:20 1.00E+06 None
Slide 2 Slide 1:20 1.00E+06 100 uL DMEM
Dish 1 Dish 1:20 1.00E+06 None
Dish 2 Dish 1:20 1.00E+06 100 uL DMEM