EricChu:LabNotesMDA/2013-9-16

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Revision as of 21:37, 17 September 2013 by >Ericchu (Created page with "== MDA Device v9 (PGP1 #16) with Tagmentation == * This device did not have a tightly closed common valve for the MDA chambers. All of the contents were leaked and evaporated...")
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MDA Device v9 (PGP1 #16) with Tagmentation[edit]

  • This device did not have a tightly closed common valve for the MDA chambers. All of the contents were leaked and evaporated through this valve.
  • No sample was collected from this experiment.

MDA Device v7 (PGP1 #15) with Tagmentation[edit]

Bead Purificaton and Size selection[edit]

  • Bead purification and Size selection were performed on 16 chosen samples in PGP1 #15 experiment.
  • Andrew's Size Selection Protocol was used. Arichard:Protocols/MDA_amplicon_size_selection
  • 130ul magnetic beads were used in bead purification (instead of 100ul beads listed in the protocol.
  • Modified protocol will be posted into my protocol page.

Library Construction[edit]

  • I've chosen samples from 16 MDA chambers and pooled two samples during size selection, based on similar intensities (2nd PCR). Each of them has a different index #.
  • 8 pools of 300-600bp were purified from TBE gel.
  • Samples and indexes are listed in the gel pictures. EricChu:LabNotesMDA/2013-9-17