EricChu:LabNotesMDA/2013-9-16
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MDA Device v9 (PGP1 #16) with Tagmentation[edit]
- This device did not have a tightly closed common valve for the MDA chambers. All of the contents were leaked and evaporated through this valve.
- No sample was collected from this experiment.
MDA Device v7 (PGP1 #15) with Tagmentation[edit]
Bead Purificaton and Size selection[edit]
- Bead purification and Size selection were performed on 16 chosen samples in PGP1 #15 experiment.
- Andrew's Size Selection Protocol was used. Arichard:Protocols/MDA_amplicon_size_selection
- 130ul magnetic beads were used in bead purification (instead of 100ul beads listed in the protocol.
- Modified protocol will be posted into my protocol page.
Library Construction[edit]
- I've chosen samples from 16 MDA chambers and pooled two samples during size selection, based on similar intensities (2nd PCR). Each of them has a different index #.
- 8 pools of 300-600bp were purified from TBE gel.
- Samples and indexes are listed in the gel pictures. EricChu:LabNotesMDA/2013-9-17