Daniel:Notebook/HiResChrPaint/2013-9-18

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Revision as of 16:29, 18 September 2013 by >Djacobse (Created page with "=Fibroblast Adherence (Started 09/09/2013)= Back to Calendar Did some research this morning on f...")
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Fibroblast Adherence (Started 09/09/2013)

Back to Calendar

Did some research this morning on fibroblast culture conditions. I found an interesting [[1]] on UCSC Genome Browser that seems to be a simple (and useful) protocol for culture. The protocol recommends four ingredients in culture medium:

  • DMEM (duh, provides all the essential nutrients)
  • Fetal Bovine Serum (has a good deal of growth factors plus lots of BSA)
  • 2-Mercaptoethanol (reduces disulfide bonds and acts as an antioxidant)
  • L-glutamine (essential amino acid frequently added to culture)

I think we can skip the L-glutamine, and probably the 2-mercaptoethanol as well. However, I think the fetal bovine serum might help out the cell culture, and as it happens Song has recently ordered some, so I'll ask him about using it.