Song Chen:Daily/NOTES/2013-9-19

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Revision as of 00:30, 20 September 2013 by >Chensong
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Aliquot Mitomycin C(Roche Cat.#10107409001)

dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 degree


Prepare Mitomycin-C-inactivated MEFs

  • [[Song:MEF_Preparation]|MEF]
  • 3*100 mm MEF(P2) --> 30 vials
  • estimated number of plated 60 mm dishes each vial: 3*107(each 100 mm dish)/30(vials)/5*105= 2