Daniel:Notebook/GenomeMiner/2013-9-20
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Mock HL155 (Started 9/9/2013)
Mimic Data, Bases 30 to 50
Last mimic data bases 30 to 50 actually put insertions/deletions from bases 1 to 29, not 30 to 50, so for good measure I'm repeating the code using the correct 30 to 50 range. This will make it directly comparable to the perfect base quality mimic data from 9/18.
Workflow
1. MockHL155_Master.m, Switch 5 2. scp v4s1mockseq_errormimic_30to50bp.fq djacobse@132.239.135.41:/media/LTS_15T/DEJ_LTS/SeqStore/130628_HL155/mockseq/errormimic_30to50/ 3. ./hl155bash.sh 4. perl imp_count_mismatch.plx
Matlab Error Counts
627618 substitutions (0.42 pct) 161680 insertions (0.11 pct) 30992 deletions (0.02 pct)
Alignment Results
2959000 reads; of these: 2959000 (100.00%) were unpaired; of these: 7858 (0.27%) aligned 0 times 2945510 (99.54%) aligned exactly 1 time 5632 (0.19%) aligned >1 times 99.73% overall alignment rate
Error Counting Results
Error Rate: 0.521% Error Rate of Insertions: 0.090% Error Rate of Deletions: 1.63e-04% Error Rate of Substitutions: 0.431%