Matt:LabNotes/2013-9-25

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Revision as of 19:18, 25 September 2013 by >Mzcai (Created page with "===Expected number of ACTB and RAB7A Rolonies=== *Initial trial of secondary gene rolony generation showed low number of rolonies http://genome-tech.ucsd.edu/LabNotes/index.p...")
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Expected number of ACTB and RAB7A Rolonies

' ID Ref ID Ref Expression Value Expression Value % of total
ACTB ILMN_2152131 ILMN_2038777 42,723.45 33,579.69 0.32
RAB7A ILMN_1716524 N/A 7,254.71 N/A 0.030
All Transcripts N/A N/A 23,976,360 N/A 100
  • According to this: http://bfg.oxfordjournals.org/content/8/6/437.full.pdf, 95% of RNA is housekeeping ncRNA. In other words ~5% of our primary rolonies is mRNA
  • Therefore expect 0.016% of primary rolonies to be ACTB secondary rolonies
  • Expect 0.0015% of primary rolonies to be RAB7A secondary rolonies