Daniel:Notebook/HiResChrPaint/2013-9-27

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Revision as of 18:32, 27 September 2013 by >Djacobse (Created page with "=FISH= Back to Calendar Using the slides from Tuesday, I'm going to do ano...")
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FISH

Back to Calendar

Using the slides from Tuesday, I'm going to do another FISH experiment.

Day 1

1. Allow stored slides to warm to room temperature
2. Incubate slides in 2X SSCT +50% (v/v) formamide for 2.5 minutes at 92 C in a pre-warmed coplin jar
3. Incubate in coplin jar containing 2X SSCT + 50% formamide at 60C for 20 minutes
4. Remove slides and allow to cool to room temperature
5. Add 25 uL hybridization cocktail to a 22x22 #1.5 coverslip
6. Invert slides on to cocktail-covered coverslips; seal with rubber cement
7. Allow the rubber cement to air-dry for 5 minutes at room temperature
8. Denature for 2.5 minutes at 92C; keep humid
9. Transfer slides to a humidified chamber and hybridize overnight at 37C or 42C