Hosuk:LabNotes/2013-9-26

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Revision as of 19:07, 27 September 2013 by >Hosuki78 (Created page with "*LabNote ===ACTB Gene detection in 1st Rolonies=== ====Procedure==== *Directly anneal ATTO488_ACTB_on1stRolony_target_Probe. *Use 06232013 sample – 3uL...")
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ACTB Gene detection in 1st Rolonies

Procedure

  • Directly anneal ATTO488_ACTB_on1stRolony_target_Probe.
  • Use 06232013 sample – 3uL Phi29 (2nd rolony experiment sample) and 10uL Phi29
  • Procedure
    1. Strip Sample dishes with 80% Formamide
    2. Prepare 1uM Alex288_ACTB_on1stRolony_target_Probe in 200uL 2x SSC
    3. Preheat the probe at 65C for 5min
    4. Add probes in dish, incubate for 10min at RT
    5. Wash, and image


  • ACTB_1stRolonyFISH : GGCAAAGGCGAGGCT/3ATTO488N/
  • Shifted FISSEQ_2ndRCAprimerDye : GGATATCGGGAAGCTGAA/3ATTO488N/