Hosuk:LabNotes/2013-9-26
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ACTB Gene detection in 1st Rolonies
Procedure
- Directly anneal ATTO488_ACTB_on1stRolony_target_Probe.
- Use 06232013 sample – 3uL Phi29 (2nd rolony experiment sample) and 10uL Phi29
- Procedure
- Strip Sample dishes with 80% Formamide
- Prepare 1uM Alex288_ACTB_on1stRolony_target_Probe in 200uL 2x SSC
- Preheat the probe at 65C for 5min
- Add probes in dish, incubate for 10min at RT
- Wash, and image
- ACTB_1stRolonyFISH : GGCAAAGGCGAGGCT/3ATTO488N/
- Shifted FISSEQ_2ndRCAprimerDye : GGATATCGGGAAGCTGAA/3ATTO488N/