Daniel:Notebook/HiResChrPaint/2013-9-30

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Revision as of 17:11, 1 October 2013 by >Djacobse (Created page with "=Fibroblast Culture= Back to Calendar Split passage 9 cells into 3 culture dishes, also prepped 5 plates fo...")
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Fibroblast Culture

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Split passage 9 cells into 3 culture dishes, also prepped 5 plates for fixing/hybridization.

Prepping cells for hybridization

1. Added 100 uL of cell suspension (after step 5) to center of petri dish
2. Incubated for ~9 hours
3. Aspirated media and added 1 mL fresh DMEM
4. Incubated overnight