Arichard:Notebook/2013/October

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Revision as of 17:20, 4 October 2013 by >Andrew (Created page with "==October, 2013== ===10/01/2013=== * Started MIDAS with Alz. neu. nuc. ID #25-00+ ===10/02/2013=== * Forgot to turn on temperature control. Overnight MDA ran at ~22 degC i...")
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October, 2013

10/01/2013

  • Started MIDAS with Alz. neu. nuc. ID #25-00+

10/02/2013

  • Forgot to turn on temperature control. Overnight MDA ran at ~22 degC instead of 30.
    • According to NEB website, Phi29 pol processes 2280 nt/min at 30 degC, and 1490 nt/min at 15 degC.
    • Based on this, I decided to continue with the experiment.
  • Spilled the tubes after adding 75% EtOH wash after precipitation.
    • Tubes 9-12 were closed already, should not have been affected.
    • Other tubes still had visible pellets, so I just added more wash and continued.

10/03/2013

File:2013 10 03 midas alz pcr1.jpg

File:2013 10 03 midas alz pcr2.jpg