Arichard:Notebook/2013/October
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October, 2013
10/01/2013
- Started MIDAS with Alz. neu. nuc. ID #25-00+
10/02/2013
- Forgot to turn on temperature control. Overnight MDA ran at ~22 degC instead of 30.
- According to NEB website, Phi29 pol processes 2280 nt/min at 30 degC, and 1490 nt/min at 15 degC.
- Based on this, I decided to continue with the experiment.
- Spilled the tubes after adding 75% EtOH wash after precipitation.
- Tubes 9-12 were closed already, should not have been affected.
- Other tubes still had visible pellets, so I just added more wash and continued.