Kun:LabNotes/ASE/2007-11-6
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Exp. 1 PAGE purification of the eSNP84k probes
Exp. 2 Continue on the second round of probe prep.
Starting from the ethanol precipitated PCR amplicons from 800ul reactions.
The DNAs were resuspended in 100ul H2O. I added 10ul 10X NEBuffer 4, 4ul BciVI, 37C 3h, 75C 10min. I have one extra tube of Lib #5, which was included in the experiment.