Hosuk:LabNotes/2013-10-9

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Revision as of 21:31, 9 October 2013 by >Hosuki78 (Created page with "*LabNote ===1st Rolonies and ACTB Gene detection in 1st Rolonies - Tile scan, 2k x 2k resolution images with Confocal === ====Parameters==== *488nm laser...")
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1st Rolonies and ACTB Gene detection in 1st Rolonies - Tile scan, 2k x 2k resolution images with Confocal

Parameters

  • 488nm laser for ACTB target probe (ATTO488), 638nm for 1st Rolonies (Cy5)
  • 20x obj., zoom = 1.00, Pinhole = 2.00
  • Resolution format : 2048 x 2048 Format,
  • Field of view : 581um x 581 um
  • Pixel size : 284nm x 284nm
  • PMT1 (ATTO488) : Gain 670V, Offset -2.00%
  • PMT2 (Cy5) : Gain 550V, Offset -2.00%
  • Z stack : step size : 0.54um, Total 10.87um scan (21 images)
  • Tile size = 8 x 6
  • Total scanning time : 3hr. 10min