Rui:10.10.13 discussion
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10.10.13 discussion[edit]
- Current methods
Efficiency Ref(10pg) SC(mNPC,mES) SN(nuclei) Smart-seq 10% + + N/A Smart-seq2 40% (est.) ILMN-dT/N6-9 ? + +(?) C1/Smart-seq 10-20% + +(?) STRT/C1 (Stenn, UMI) 48% Cel-seq 17%
- Methods plan
# STRT/C1 integrated with frag./PAP # Cel-seq integrated with frag./PAP # fine tune PAP: control length # different T20/T30 primers: SEvsPE, T20/T30, VN-V-none
- Upstream
# cell sorting # C1 fluidigm # LCM
- Downstream
# data analysis: TSCC, Kun's scripts # Wei's group