Song Chen:Daily/NOTES/2013-10-16

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Revision as of 21:28, 17 October 2013 by >Chensong
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FACS sorting

  • Place:UCSD hESC Core
  • Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
  • Brief procedure:
  1. Dissociate culture into single cells
  2. Wash twice with PBS
  3. Filter cells with cell strainer
Suspend cells best in 106/ml density
Place cell suspension in ice(3rd floor)
Bring culture medium to collect sorted cells


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