Hosuk:LabNotes/2013-10-17

From ZhangLabWiki
Revision as of 04:35, 18 October 2013 by >Hosuki78 (Created page with "*LabNote ===Rolonies with fresh cells (96well plate)=== ====Goal==== *Produce rolonies with (relatively) fresh cells *Try invitrogen a-dUTP (from Dan) ...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search


Rolonies with fresh cells (96well plate)

Goal

  • Produce rolonies with (relatively) fresh cells
  • Try invitrogen a-dUTP (from Dan) and compare to the current (brown vial) anaspect's one


Process

  • Got PGP1F from Alan, the cells were thaw two days ago
  • Split cells (10/16) on 8 wells in 96 well plate, 2 Mattek dishes
  • Fix cells (10/17) and proceed RT (9:30 pm)