Hosuk:LabNotes/2013-10-19
Jump to navigation
Jump to search
Rolonies with fresh cells (96well plate)
Process
- Start (10/17)
- End RCA : 1:30pm 10/19
Result
- Not good : there are rolonies, but almost no ACTB
- Cells in 6 out of 8 wells don’t look good, they were detached, less cells in wells.. WHY? Something happened when fixing?
- Cells were aggregated or became chunk… need to mix when split
- Fixing two step : 37% Formalin 10% Formalin
- The very first protocol was two step fixing, but Jonathan has been doing one step (10% Formalin), and in their manuscript as well.
- But… I want to try two step fixing and compare to one step fixing…