Hosuk:LabNotes/2013-10-21

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Revision as of 00:34, 22 October 2013 by >Hosuki78 (Created page with "*LabNote ===Fixing cells in 96 well plate=== *I've split cells in 16 wells. *First 8 wells are fixed with 2 step, others with 1 step fixing. *For 1 stp...")
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Fixing cells in 96 well plate

  • I've split cells in 16 wells.
  • First 8 wells are fixed with 2 step, others with 1 step fixing.


  • For 1 stpe fixing
    • Take out 150uL media, so there is 150uL in well
    • Add 100uL 10% formalin, so it is 4% formalin in well.


  • For 2 step fixing
    • Take out 100uL media, so there is 200#uL in well
    • Add 20uL 37% formalin, so it is 1:10 ratio, as following the first rolony protocol that I've got.