Daniel:Notebook/HiResChrPaint/2013-10-22

From ZhangLabWiki
Revision as of 01:05, 23 October 2013 by >Djacobse (→‎BSPP Control)
Jump to navigation Jump to search

BSPP Control

Back to Calendar

  • For first production of BSPP control, see 09/03/2013

Since I'm out of control group, I'm going to make some more.

qPCR

1. Reaction Table
Sample BSPP 1A BSPP 1B BSPP 1C BSPP 1D BSPP 2A BSPP 2B BSPP 2C BSPP 2D
ng/uL 97.9 94.5 96.9 91.9 90.2 92.5 90.7 82.4
ug in 35 uL 3.4 3.3 3.4 3.2 3.2 3.2 3.2 2.9
uL 10X buffer 5 5 5 5 5 5 5 5
uL DpnII (50U/uL) 2 2 2 2 2 2 2 2
uL nfH2O 8 8 8 8 8 8 8 8
Total 50 50 50 50 50 50 50 50
2. Incubate samples at 37C for 16 hours; 20 minute heat shock at 65C; Continued tomorrow