Hosuk:LabNotes/2013-10-22
Jump to navigation
Jump to search
Rolonies with fresh cells - MatTek dish
- Produce rolonies in MatTek dishes which were fixed at 10/17, the same cells to 96 well plate.
Process
- 2 dishes
- RT : 8:30 pm
- CircLigase : 10/21 12:00 pm
- End RCA : 10/22 13:00pm
- Imaging
Result
- Good, many rolonies, and more ACTB than before.
- Of course, the number and density of rolonies are still not as many as the sample 06/23...
- Doing Confocal
- Doing 1 location as well as tile scanning
- Some of pictures...
- location 1 : 1st Rolonies, ACTB
- location 2 : 1st Rolonies, ACTB