Hosuk:LabNotes/2013-10-22

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Revision as of 23:37, 22 October 2013 by >Hosuki78 (Created page with "===Rolonies with fresh cells - MatTek dish=== *Produce rolonies in MatTek dishes which were fixed at 10/17, the same cells to 96 well plate. ====Process==== *2 dishes *RT :...")
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Rolonies with fresh cells - MatTek dish

  • Produce rolonies in MatTek dishes which were fixed at 10/17, the same cells to 96 well plate.

Process

  • 2 dishes
  • RT : 8:30 pm
  • CircLigase : 10/21 12:00 pm
  • End RCA : 10/22 13:00pm
  • Imaging


Result

  • Good, many rolonies, and more ACTB than before.
    • Of course, the number and density of rolonies are still not as many as the sample 06/23...
  • Doing Confocal
    • Doing 1 location as well as tile scanning